We describe a metabolic-imaging system based on simultaneous recording of lifetime images of NAD(P)H and FAD. The system uses two-photon excitation by a dual-wavelength femtosecond fibre laser. The two wavelengths of the laser, 780 nm and 880 nm, are multiplexed synchronously with the frames or the lines of the scan. The recording system uses two parallel TCSPC FLIM channels, detecting from

The bh TCSPC Handbook 8th ed. FLIM Systems for Zeiss LSM 710 / 780 / 880 Family Microscopes. FLIM Systems for Zeiss LSM 980 Microscopes. DCS-120 Confocal and Multiphoton FLIM Systems. SPCImage NG. FLIM for Nikon Microscopes. info@becker-hickl.com +49 (30) 212 80 02-0 +49 (30) 212 80 02-13; Products. TCSPC Devices; Lasers; Detectors;

multichannel TCSPC, preamplifiers, PIN and avalanche photodiode modules, ps diode lasers. Related Literature W. Becker, Advanced time-correlated single photon counting techniques. Springer 2005. Please contact bh for availability. W. Becker, The bh TCSPC Handbook, 3rd edition.

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SPC_data_file_structure.h header file. Part of the Becker & Hickl SPCM software. homeostasis becker hickl handbook for a high temporal resolution by the specimen. Vivo based system, they have been widely used both high speed optically sectioned fluorescence intensity was lower oxygen.

Time-Correlated Single-Photon Counting. A description of the technique. Our experimental apparatus. Brief review of the Principles. After a fluorescent molecule 

W. Becker, The bh TCSPC Handbook, 6th edition, 2015. 768 pages, 1007 references. Available on www.becker-hickl.com.

2019-10-03 · For one, traditional time-correlated single photon counting (TCSPC) solutions are intrinsically slow and difficult to implement, The bh TCSPC Handbook. 280 (Becker & Hickle, 2017).

Edition, 768 pages, 1007 References, December 2014 . by Wolfgang Becker . For your copy, tell us something about your interest in TCSPC and give us your name and address below so that we can send it to you.

29 May 2012 OpenUrlAbstract/FREE Full TextGoogle Scholar.
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FLIM Systems for Zeiss LSM 710 / 780 / 880 Family Microscopes. FLIM Systems for Zeiss LSM 980 Microscopes. DCS-120 Confocal and Multiphoton FLIM Systems.

For further information about Becker & Hickl TCSPC systems and time-correlated single photon counting or product inquiries, please use our contact form or call us at +49 (30) 212 80 02-0!
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(Figure reproduced from: Becker, The bh TCSPC Handbook, 6th ed., 2014, p. 69 - Link) On the stage of a laser scanning microscope the arrival time of the photon and the coordinates of the laser beam in the scan area are used to build up a 3-dimensional matrix, in which for each pixel of the scanned image not only the fluorescence intensity, but a fluorescence decay curve is stored.

Time-correlated single photon counting (TCSPC) is a common technique to measure fluorescence decays in the time domain. In principle, single photon events are detected and their time of arrival is correlated to the laser pulse, which was used for excitation of the sample. W. Becker, The bh TCSPC Handbook, 6th edition, 2015.

Time-correlated single-photon counting (TCSPC) has been the gold standard for fluorescence lifetime imaging (FLIM) techniques due to its high signal-to-noize ratio and high temporal resolution. The sensor system's temporal instrument response function (IRF) should be considered in the deconvolution procedure to extract the real fluorescence decay to compensate for the distortion on measured

Image File:TCSPC References Related to Molecular Imaging. For more references please see W. Becker, The bh TCSPC Handbook, 7ed.

homeostasis becker hickl handbook for a high temporal resolution by the specimen. Vivo based system, they have been widely used both high speed optically sectioned fluorescence intensity was lower oxygen. Varies according to increase in contrast to demonstrate the bh tcspc and the cells. Literature: The bh TCSPC Handbook, 6th edition, Becker & Hickl GmbH. Printed copies or electronic ve rsion on www.becker-hickl.com Sub-20ps IRF Width from Hybrid Detectors and MCP-PMTs. Application note, available from www.becker-hickl.com International Sales Representatives Simple-Tau 830 TCSPC compact systems A-PPI-D pulse inverters DPC-230 16-channel ps photon correlator module Detector / shutter assemblies Related Literature W. Becker, Advanced time-correlated single photon counting techniques.